ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using Act5C antibody against A549(1) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ICC/IF
Immunofluorescence analysis of A549 cells using Act5C antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-Act5C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | DrosophilaMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Act5C |
|---|---|
| Gene Synonyms | 0610041G09Rik A a-SMA A4V404_DROME Ac5C ACT act 5C Act-4 ACT-5 Act-5C ACT1_DROME Act4 Act5 ACT5C ACTA Acta-2 ACTA1 ACTA3 ACTC Actc-1 ACTE ACTG ACTGE Actin Actin/BAP47 Actin5C Actl ACTL3 Acts ACTSA ACTSG Actsk-1 Actvs Actx alphaSMA anon-EST:fe2D2 ArpA ASD5 ASMA BAP47 beta-actin beta-actin/Bap47 BKRNS BNS BRWS1 CFTD CFTD1 CFTDM CG4027 chrX:5748184..5748304 CMD1R CMH11 CMYO2A CMYO2B CMYO2C CMYP2A CMYP2B CMYP2C CSMH cyt5C DDS1 DFNA20 DFNA26 Dmel\CG4027 Dmel_CG4027 E430023M04Rik E51 F-actin G-actin HEL-176 l(1)G0009 l(1)G0010 l(1)G0025 l(1)G0079 l(1)G0117 l(1)G0177 l(1)G0245 l(1)G0330 l(1)G0420 l(1)G0486 LVNC4 M32055 MMIHS5 MPFD NEM1 NEM2 NEM3 PS1TP5BP1 SHPM SMAalpha SMalphaA SMDYS SMGA T11 THC8 VSCM VSCM1 |
| Gene Full Name | Actin 5C |
| Gene Summary | Involved in several processes, including germ cell development; maintenance of protein location in cell; and mitotic cytokinesis. Part of Ino80 complex and brahma complex. Is expressed in several structures, including adult fat body; adult heart; ganglia; gut section; and sensory nervous system primordium. Human ortholog(s) of this gene implicated in Baraitser-Winter syndrome 1; Baraitser-Winter syndrome 2; autosomal dominant nonsyndromic deafness 20; and thrombocytopenia. Orthologous to several human genes including ACTB (actin beta) and ACTG1 (actin gamma 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 5C actin A-X actin A-X actin-like protein ACT2 Act5C-PA Act5C-PB Act5C-PC Act5C-PD Act5C-PE ACTA1 ACTA2 ACTC ACTG2 actin actin 2 actin 5 C actin 5c actin 7 actin A1 actin alpha 1 actin alpha cardiac Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments which subsequently assemble into interconnected networks within the cytoplasm of cells. actin alpha 1 skeletal muscle actin alpha 2 smooth muscle aorta Actin alpha skeletal muscle actin alpha cardiac 1 actin alpha vascular smooth muscle actin beta cytoplasmic actin gamma 2 smooth muscle enteric actin gamma cytoplasmic 1 actin-like protein actin5C alpha-actin alpha-actin cardiac Alpha-actin-1 alpha-actin-2 alpha-actin-3 alpha-cardiac actin alpha-sma alpha-smooth muscle actin alphac-actin anon-fast-evolving-2D2 AtACT7 Atcb beta cytoskeletal actin beta-actin beta-actin FE-3 cardiac actin cell growth-inhibiting gene 46 protein cellular cytoskeletal beta-actin CG4027-PA CG4027-PB CG4027-PC CG4027-PD CG4027-PE cytoplasmic beta-actin cytoplasmic protein cytoskeletal gamma-actin DEFORMED ROOT HAIRS 1 DER1 Dm-actin5C ENHANCER OF LRX1 2 ENL2 epididymis luminal protein 176 F-actin FIZ2 FRIZZY AND KINKED SHOOTS 2 G-actin gamma non-muscle actin gamma-2-actin gamma-actin gamma-enteric smooth muscle actin GIG46 I(2)-actin LIGHT STRESS-REGULATED 2 LSR2 melanoma X-actin MYH9.2 MYH9_2 nemaline myopathy type 3 pan actin PS1TP5-binding protein 1 RE02927p RP5-1068B5.2 skeletal muscle alpha-actin smooth muscle alpha-actin smooth muscle gamma-actin |
| Molecular Weight(MW) | 42kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, cytoskeleton |
| SwissProt ID | P10987 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using Act5C antibody against A549(1) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ICC/IF
Immunofluorescence analysis of A549 cells using Act5C antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
