ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.ICC/IF
Immunofluorescence analysis of Hela cells using PIK3R2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-PIK3R2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ELISA:1:1000-1:10000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3R2 |
|---|---|
| Gene Synonyms | AGM7 GRB1 IMD36 MPPH MPPH1 p50alpha p55alpha p85 p85-ALPHA p85-BETA p85alpha P85B p85beta PI3K PI3KA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 2 |
| Gene Summary | Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene. [provided by RefSeq, Apr 2019] |
| Alternative Names | growth factor receptor bound 1 p85-alpha GRB1 phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta phosphatidylinositol 3-kinase p85 alpha phosphatidylinositol 3-kinase regulartory subunit polypeptide 2 phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphatidylinositol 3-kinase regulatory subunit polypeptide 2 (p85 beta) phosphatidylinositol 3-kinase-associated p-85 alpha Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50) Phosphoinositide 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphoinositide-3-kinase regulatory subunit alpha phosphoinositide-3-kinase regulatory subunit beta phosphoinositide-3-kinase regulatory subunit 1 (alpha) phosphoinositide-3-kinase regulatory subunit 2 (beta) PI3 kinase-associated p85 PI3-kinase p85 subunit alpha PI3-kinase p85 subunit beta PI3-kinase regulatory subunit alpha PI3-kinase regulatory subunit beta PI3-kinase subunit p85-alpha PI3-kinase subunit p85-beta PI3K regulatory subunit alpha PI3K regulatory subunit beta PI3K-p46 ptdIns-3-kinase p85-alpha ptdIns-3-kinase p85-beta ptdIns-3-kinase regulatory subunit alpha ptdIns-3-kinase regulatory subunit beta ptdIns-3-kinase regulatory subunit p85-alpha ptdIns-3-kinase regulatory subunit p85-beta |
| Molecular Weight(MW) | 81kDa |
| Source | Rabbit |
| Cellular Localization | cytosol;focal adhesion;nucleus; |
| SwissProt ID | O00459 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
ICC/IF
Immunofluorescence analysis of Hela cells using PIK3R2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ELISA:1:1000-1:10000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
