ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using KCNN4 antibody against Jurkat(1),C2C12(2),P19(3),Raji(4),Pc-12(5) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-KCNN4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KCNN4 |
|---|---|
| Gene Synonyms | DHS2 hIK1 hIKCa1 hKCa4 hSK4 IK IK1 IKCA1 KCa3.1 KCA4 mIK1 mIKCa1 mKCa4 SK4 SKCa4 SKCas |
| Gene Full Name | potassium calcium-activated channel subfamily N member 4 |
| Gene Summary | The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008] |
| Alternative Names | gardos channel HIK1 IKCa intermediate conductance calcium-activated potassium channel intermediate conductance calcium-activated potassium channel protein 1 intermediate-conductance Ca2+-activated K+ channel KCa3.1 KCNN 4 KCNN4 mKCa4 KCNN4 Mutations: 960:OK potassium channel calcium activated intermediate/small conductance subfamily N alpha member 4 potassium intermediate/small conductance calcium-activated channel subfamily N member 4 putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel putative Gardos channel SKCa 4 SKCa4 small conductance calcium-activated potassium channel 4 |
| Molecular Weight(MW) | 47kDa（observed：40kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane;Cell projection, ruffle membrane |
| SwissProt ID | O15554 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using KCNN4 antibody against Jurkat(1),C2C12(2),P19(3),Raji(4),Pc-12(5) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
