ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using FCGRT antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),THP-1(5),A549(6) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-FCGRT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FCGRT |
|---|---|
| Gene Synonyms | alpha-chain FcgammaRn FCRN |
| Gene Full Name | Fc gamma receptor and transporter |
| Gene Summary | This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| Alternative Names | Fc fragment immunoglobulin G receptor Fc fragment of IgG receptor and transporter Fc fragment of IgG receptor transporter alpha Fc receptor IgG alpha chain transporter FcRn alpha chain heavy chain of the major histocompatibility complex class I-like Fc receptor IgG Fc fragment receptor transporter alpha chain IgG Fc region receptor large subunit p51 (FcRn) precursor immunoglobulin receptor intestinal heavy chain major histocompatibility complex class I-like Fc receptor neonatal Fc receptor neonatal Fc-receptor for Ig neonatal fragment crystallizable Fc receptor neonatal fragment crystallizable Fc receptor FcRn transmembrane alpha chain of the neonatal receptor |
| Molecular Weight(MW) | 39kDa（observed：40kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane；Endosome membrane |
| SwissProt ID | P55899 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using FCGRT antibody against Hela(1),Cos-7(2),K562(3),Hepg2(4),THP-1(5),A549(6) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
