ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using HTR1F antibody against Hela(1),Hepg2(2),A549(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-HTR1F antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | HTR1F |
|---|---|
| Gene Synonyms | 5-HT-1F 5-HT1F 5HT6 Htr1eb HTR1EL MR77 |
| Gene Full Name | 5-hydroxytryptamine receptor 1F |
| Gene Summary | Enables Gi/o-coupled serotonin receptor activity; serotonin binding activity; and serotonin receptor activity. Involved in adenylate cyclase-inhibiting serotonin receptor signaling pathway. Located in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 5-HT-1E-beta 5-HT-1F 5-HT1F 5-HT1f receptor 5-hydroxytryptamine (serotonin) receptor 1E beta 5-hydroxytryptamine (serotonin) receptor 1F G protein-coupled 5HT1F Receptor serotonin receptor serotonin receptor 1F serotonin receptor gene (similar to Mm Htr1eb) |
| Molecular Weight(MW) | 41kDa |
| Source | Rabbit |
| Cellular Localization | Cell membrane |
| SwissProt ID | P30939 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using HTR1F antibody against Hela(1),Hepg2(2),A549(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
