ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using IDO2 antibody against B16(1),Nih3t3(2) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.| Product Name | Anti-IDO2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IDO2 |
|---|---|
| Gene Synonyms | C230043N17Rik Ido-2 INDOL1 |
| Gene Full Name | indoleamine 2,3-dioxygenase 2 |
| Gene Summary | Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011] |
| Alternative Names | 3-dioxygenase 2 3-dioxygenase-like protein 1 EC 1.13.11. INDOL 1 indoleamine 2 Indoleamine 2 3 dioxygenase 2 Indoleamine 2 3 dioxygenase like 1 protein indoleamine 2 3-dioxygenase-like 1 protein indoleamine 2 3-dioxygenase-like protein 1 Indoleamine pyrrole 2 3 dioxygenase like 1 Indoleamine-pyrrole 2 indoleamine-pyrrole 2 3 dioxygenase-like 1 indoleamine-pyrrole 2 3-dioxygenase indoleamine-pyrrole 2 3-dioxygenase-like protein 1 |
| Molecular Weight(MW) | 45kDa |
| Source | Rabbit |
| Cellular Localization | cytoplasm;cytosol;Complete GO annotation on QuickGO |
| SwissProt ID | Q6ZQW0 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using IDO2 antibody against B16(1),Nih3t3(2) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
