ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using SCNN1G antibody against Hela(1),Cos-7(2) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-SCNN1G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCNN1G |
|---|---|
| Gene Synonyms | BESC3 ENaC ENaCg ENaCgamma I79_020711 LDLS2 PHA1 PHA1B3 SCNEG |
| Gene Full Name | sodium channel epithelial 1 subunit gamma |
| Gene Summary | Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the gamma subunit, and mutations in this gene have been associated with Liddle syndrome. [provided by RefSeq, Apr 2009] |
| Alternative Names | amiloride sensitive sodium channel gamma1 subunit amiloride-sensitive epithelial sodium channel gamma subunit amiloride-sensitive sodium channel gamma-subunit amiloride-sensitive sodium channel subunit gamma ENaC gamma ENaC gamma subunit ENaC subunit gamma epithelial Na(+) channel subunit gamma epithelial sodium channel gamma subunit Gamma ENaC Gamma NaCH gamma-ENaC gamma-NaCH ion channel nasal ENaC hnENac nonvoltage-gated sodium channel 1 subunit gamma PHA 1 pithelial Na(+) channel subunit gamma SCNEG SCNN 1G sodium channe epithelial 1 gamma subunit sodium channel epithelial 1 gamma subunit sodium channel non-voltage-gated 1 gamma subunit sodium channel nonvoltage-gated 1 gamma sodium channel nonvoltage-gated 1 gamma Sodium channel nonvoltage-gated 1 gamma (epithelial) sodium channel nonvoltage-gated type I gamma |
| Molecular Weight(MW) | 74kDa |
| Source | Rabbit |
| Cellular Localization | Apical cell membrane |
| SwissProt ID | P51170 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using SCNN1G antibody against Hela(1),Cos-7(2) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
