ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using CD32 antibody against B16(1) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-CD32 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FCGR2C |
|---|---|
| Gene Synonyms | CD32 CD32A CD32B CD32C CDW32 FCG2 FcgammaRIIa FcgammaRIIc FcGR FCGR2 FCGR2A1 FCRIIC IGFR2 |
| Gene Full Name | Fc gamma receptor IIc (gene/pseudogene) |
| Gene Summary | This gene encodes one of three members of a family of low-affinity immunoglobulin gamma Fc receptors found on the surface of many immune response cells. The encoded protein is a transmembrane glycoprotein and may be involved in phagocytosis and clearing of immune complexes. An allelic polymorphism in this gene results in both coding and non-coding variants. [provided by RefSeq, Apr 2012] |
| Alternative Names | CD32 receptor 2 CD32 receptor 2a CDw32 Fc fragment of IgG receptor IIa Fc fragment of IgG receptor IIb Fc fragment of IgG receptor IIc Fc fragment of IgG low affinity II receptor for (CD32) Fc fragment of IgG low affinity IIa receptor (CD32) Fc fragment of IgG low affinity IIb receptor (CD32) Fc fragment of IgG low affinity IIb receptor for (CD32) Fc fragment of IgG low affinity IIc receptor for (CD32) Fc gamma receptor IIC Fc gamma receptor RIIa3 Fc gamma RIIb fc-gamma RII-b fc-gamma-RIIa fc-gamma-RIIb fc-gamma-RIIc FCGR2B FcgRIIa3 splice variant fcRII-a fcRII-b FGFR2B IgG Fc fragment receptor IgG Fc fragment receptor precursor IgG Fc receptor alpha-Fc-gamma-RII IgG Fc receptor beta-Fc-gamma-RII igG Fc receptor II-a igG Fc receptor II-b IgG Fc receptor II-c IgG Fc receptor precursor Immunoglobulin G Fc receptor II immunoglobulin G Fc receptor II-c MGC23887 MGC30032 RP11-5K23.6 |
| Molecular Weight(MW) | 35kDa（observed：37kDa) |
| Source | Rabbit |
| Cellular Localization | Cytoplasm;Cell membrane |
| SwissProt ID | P31995 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using CD32 antibody against B16(1) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
