ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using SLC9A1 antibody against Hela(1),K562(2),B16(3),Hepg2(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-SLC9A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC9A1 |
|---|---|
| Gene Synonyms | APNH LIKNS mir-5122 Mir5122 NHE-1 NHE1 PPP1R143 swe |
| Gene Full Name | solute carrier family 9 member A1 |
| Gene Summary | This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011] |
| Alternative Names | Na(+)/H(+) exchanger 1 Na+/H+ antiporter Na+/H+ amiloride sensitive Na-Li countertransporter NHE-1 plasma membrane antiporter hemopoietic NHE1 APNH protein phosphatase 1 regulatory subunit 143 slow-wave epilepsy Solute carrier family 9 (sodium/hydrogen exchanger 1) antiporter Na+/H+ (amiloride sensitive) solute carrier family 9 (sodium/hydrogen exchanger) member 1 solute carrier family 9 (sodium/hydrogen exchanger) member 1 (antiporter Na+/H+ amiloride sensitive) solute carrier family 9 member 1 solute carrier family 9 member 1 solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 |
| Molecular Weight(MW) | 90kDa（observed：45kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane;Basolateral cell membrane |
| SwissProt ID | P19634 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using SLC9A1 antibody against Hela(1),K562(2),B16(3),Hepg2(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
