ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using PDE4D antibody against Hela(1),Cos-7(2),K562(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-PDE4D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDE4D |
|---|---|
| Gene Synonyms | 9630011N22Rik ACRDYS2 DPDE3 HSPDE4D PDE3/IVd PDE43 PDE4DN2 STRK1 |
| Gene Full Name | phosphodiesterase 4D |
| Gene Summary | This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009] |
| Alternative Names | 3' 5'-cyclic AMP phosphodiesterase alternatively spliced cAMP-specific 3' 5'-cyclic phosphodiesterase 4D cAMP-specific phosphodiesterase 4D cAMP-specific phosphodiesterase PDE4D cAMP-specific phosphodiesterase PDE4D6 cyclic AMP specific phosphodiesterase PDE4D5A DKFZp686M11213 dunce FLJ97311 HGNC:8783 HS PDE4DN2 PDE4D1 PDE4D10 PDE4D11 PDE4D2_v1 PDE4D2_v2 PDE4D2_v3 PDE4D3 PDE4D6 PDE4D7 phosphodiesterase 4D cAMP-specific (phosphodiesterase E3 dunce homolog Drosophila) splice variant PDE4D4 testicular tissue protein Li 136 |
| Molecular Weight(MW) | 91kDa（observed：70/95/120kDa) |
| Source | Rabbit |
| Cellular Localization | Apical cell membrane;Cytoplasm;Membrane;Cytoplasm, cytoskeleton;Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| SwissProt ID | Q08499 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using PDE4D antibody against Hela(1),Cos-7(2),K562(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
