WB
Western blot analysis of Phospho-c-Jun (S73)+JunD (S100) on different lysates with Rabbit anti-Phospho-c-Jun (S73)+JunD (S100) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 25μg/mL anisomycin for 30 minutes cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells untreated / treated with 250ng/mL anisomycin for 30 minutes labeling Phospho-c-Jun (S73)+JunD (S100) with Rabbit anti-Phospho-c-Jun (S73)+JunD (S100) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-c-Jun (S73)+JunD (S100) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L ( 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594 ) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HeLa cells untreated (left) / treated with 250ng/mL anisomycin for 30 minutes (right) labeling Phospho-c-Jun (S73)+JunD (S100). Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Phospho-c-Jun (S73)+JunD (S100) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun. |
| Modification | p-S73+S100 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000 FC:1:1000 ICC/IF:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | JUN |
|---|---|
| Gene Synonyms | AP1 p39 AP-1 cJUN c-Jun |
| Gene Full Name | Jun proto-oncogene, AP-1 transcription factor subunit |
| Gene Summary | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 40/45 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P05412 |
|---|
WB
Western blot analysis of Phospho-c-Jun (S73)+JunD (S100) on different lysates with Rabbit anti-Phospho-c-Jun (S73)+JunD (S100) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 25μg/mL anisomycin for 30 minutes cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells untreated / treated with 250ng/mL anisomycin for 30 minutes labeling Phospho-c-Jun (S73)+JunD (S100) with Rabbit anti-Phospho-c-Jun (S73)+JunD (S100) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-c-Jun (S73)+JunD (S100) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L ( 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594 ) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa cells untreated (left) / treated with 250ng/mL anisomycin for 30 minutes (right) labeling Phospho-c-Jun (S73)+JunD (S100). Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 FC:1:1000 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
