WB
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate All lanes: CAND1 antibody at 4.9µg/ml.Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of CAND1 Antibody diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.IHC
IHC image of CAND1 Antibody diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of A549 cells with CAND1 Antibody at 1:100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-CAND1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Cullin-associated NEDD8-dissociated protein 1 protein (1150-1230AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAND1 |
|---|---|
| Gene Synonyms | TIP120 TIP120A |
| Gene Full Name | cullin associated and neddylation dissociated 1 |
| Gene Summary | This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016] |
| Alternative Names | CAND 1 antibody Cand1 antibody CAND1_HUMAN antibody Cullin associated and neddylation dissociated 1 antibody Cullin associated and neddylation dissociated protein 1 antibody Cullin associated NEDD8 dissociated protein 1 antibody Cullin-associated and neddylation-dissociated protein 1 antibody Cullin-associated NEDD8-dissociated protein 1 antibody DKFZP434M1414 antibody FLJ10114 antibody FLJ10929 antibody FLJ38691 antibody FLJ90441 antibody KIAA0829 antibody p120 CAND1 antibody TATA binding protein interacting protein 120A antibody TBP interacting protein antibody TBP interacting protein of 120 kDa A antibody TBP Interacting Protein TIP120A antibody TBP-interacting protein 120A antibody TBP-interacting protein of 120 kDa A antibody TIP120 antibody TIP120 protein antibody TIP120A antibody |
| Molecular Weight(MW) | 137 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate All lanes: CAND1 antibody at 4.9µg/ml.Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of CAND1 Antibody diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
IHC image of CAND1 Antibody diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of A549 cells with CAND1 Antibody at 1:100, counterstained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
