WB
Positive WB detected in: K562 whole cell lysate All lanes: CENPJ antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilutionICC/IF
Immunofluorescence staining of HepG2 cells with CENPJ Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | CBX7 RabCENPJ Antibodybit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Centromere protein J protein (1082-1248AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:5000 ICC/IF:1:200-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CPAP |
|---|---|
| Gene Synonyms | LAP LIP1 BM032 CENPJ MCPH6 SASS4 SCKL4 Sas-4 CENP-J |
| Gene Full Name | centrosome assembly and centriole elongation protein |
| Gene Summary | This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012] |
| Alternative Names | BM032 antibody CENP-J antibody CENPJ antibody CENPJ_HUMAN antibody Centromere protein J antibody Centrosomal P4.1-associated protein antibody CPAP antibody LAG-3-associated protein antibody LAP antibody LIP1 antibody LYST interacting protein LIP1 antibody LYST interacting protein LIP7 antibody LYST-interacting protein 1 antibody MCPH6 antibody Sas 4 antibody SASS4 antibody SCKL4 antibody |
| Molecular Weight(MW) | 125 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. |
WB
Positive WB detected in: K562 whole cell lysate All lanes: CENPJ antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution
ICC/IF
Immunofluorescence staining of HepG2 cells with CENPJ Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 ICC/IF:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
