WB
Lane 1: Skeletal muscle (Rat) Lysate at 40 ug Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti-TNMD at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionWB
Lane 1: Muscle (Rat) Lysate at 40 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: SW480 (Human) Cell Lysate at 30 ug Primary: Anti- TNMD at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionIHC
Paraformaldehyde-fixed, paraffin embedded (Human mammary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IF-P
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNMD Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.IF-P
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNMD Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.IF-P
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNMD Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.| Product Name | TNMD Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human TNMD: 151-250/317. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TNMD |
|---|---|
| Gene Synonyms | TEM CHM1L BRICD4 |
| Gene Full Name | tenomodulin |
| Gene Summary | This gene encodes a protein that is related to chondromodulin-I, which is a cartilage-specific glycoprotein that functions to stimulate chondrocyte growth and to inhibit tube formation of endothelial cells. This protein is also an angiogenesis inhibitor. Genetic variation in this gene is associated with a risk for type 2 diabetes, central obesity and serum levels of systemic immune mediators in a body size-dependent manner. This gene is also a candidate gene for age-related macular degeneration, though a direct link has yet to be demonstrated. [provided by RefSeq, Sep 2009] |
| Alternative Names | BRICD4 CHM1L TEM 1110017I01Rik TNMD_HUMAN TNMD hTeM Chondromodulin-1-like protein (ChM1L | hChM1L) Chondromodulin-I-like protein Myodulin Tendin TNMD_MOUSE mTeM Chondromodulin-1-like protein (ChM1L | mChM1L) TNMD_RAT rTeM Chondromodulin-1-like protein (ChM1L | rChM1L) tenomodulin BRICHOS domain containing 4 |
| Molecular Weight(MW) | 37 kDa(Observed band size: 37 kD) |
| Cellular Localization | Membrane; Single-pass type II membrane protein. |
WB
Lane 1: Skeletal muscle (Rat) Lysate at 40 ug Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug Primary: Anti-TNMD at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
WB
Lane 1: Muscle (Rat) Lysate at 40 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: Heart (Rat) Lysate at 40 ug Lane 4: SW480 (Human) Cell Lysate at 30 ug Primary: Anti- TNMD at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
IHC
Paraformaldehyde-fixed, paraffin embedded (Human mammary gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNMD ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IF-P
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNMD Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNMD Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TNMD Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
