WB
Various lysates were subjected to SDS PAGE followed by western blot with FOXP4 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using FOXP4 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using FOXP4 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-FOXP4 (4ug; Detection:1:300) with mouse lung tissue lysate 4000ug.FC
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FOXP4 and 488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | FOXP4 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human FOXP4 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:3000 IHC:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 550/ml |
| Purification | Affinity purified |
| Gene Symbol | FOXP4 |
|---|---|
| Gene Synonyms | hFKHLA |
| Gene Full Name | forkhead box P4 |
| Gene Summary | This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Many members of the forkhead box gene family, including members of subfamily P, have roles in mammalian oncogenesis. This gene may play a role in the development of tumors of the kidney and larynx. Alternative splicing of this gene produces multiple transcript variants, some encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | FKHLA forkhead box P4 Forkhead box protein P4 FOXP4 hFKHLA |
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with FOXP4 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using FOXP4 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using FOXP4 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOXP4 antibody at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-FOXP4 (4ug; Detection:1:300) with mouse lung tissue lysate 4000ug.
FC
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FOXP4 and 488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:500-1:3000 IHC:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
