ICC/IF
Tissue/cell: HUVEC cell; 4% Paraformaldehydefixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum) at 37°C for 20 min; Incubation: AntiCDKN1A/P21 Polyclonal Antibody, conjugated 1:50, 2 hours at 37°C; DAPI (blue) was used to stain the cell nuclei.| Product Name | CDKN1A/P21 Rabbit pAb, BF647 conjugated |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human P21 : 21-100/164 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| WB:1:100-1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDKN1A |
|---|---|
| Gene Synonyms | P21 CIP1 SDI1 WAF1 CAP20 CDKN1 MDA-6 p21CIP1 |
| Gene Full Name | cyclin dependent kinase inhibitor 1A |
| Gene Summary | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | Activating Fragment 1 CAP20 Cation chloride cotransporter-interacting protein 1 CDK Interacting Protein 1 CDK-interacting protein 1 CDKI CDKN 1 CDKN1 CDKN1A CIP1 Cyclin Dependent Kinase Inhibitor 1A Cyclin-dependent kinase inhibitor 1 Cyclin-dependent kinase inhibitor 1A(P21) Cyclin-dependent kinase inhibitor 1A(p21 Cip1) DNA Synthesis Inhibitor MDA 6 MDA-6 MDA6 Melanoma Differentiation Associated Protein 6 Melanoma differentiation-associated protein 6 Melanoma differentiation-associated protein p21 P21 protein p21CIP1 p21WAF PIC1 SDI1 SLC12A9 WAF1 Wildtype p53 Activating Fragment 1 Wildtype p53-activated fragment 1 CDN1A_HUMAN. |
| Molecular Weight(MW) | 18 kDa |
| Function | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. |
| Cellular Localization | Cytoplasmic and Nuclear. |
ICC/IF
Tissue/cell: HUVEC cell; 4% Paraformaldehydefixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal rabbit serum) at 37°C for 20 min; Incubation: AntiCDKN1A/P21 Polyclonal Antibody, conjugated 1:50, 2 hours at 37°C; DAPI (blue) was used to stain the cell nuclei.| Application Notes | WB:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
