WB
Western blot analysis of various lysates using PYGB pAb at 1:1000 dilution.WB
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg PYGB antibody. Western blot was performed from the immunoprecipitate using PYGB antibody at a dilution of 1:1000.ICC/IF
Immunofluorescence analysis of HepG2 cells using PYGB pAb at dilution of 1:100 (40x lens).| Product Name | PYGB polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Multifunctional protein involved in the transcription and replication of viral RNAs. Contains the proteinases responsible for the cleavages of the polyprotein. |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 544-843 of human PYGB (NP_002853.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:500-1:2000 IP:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PYGB |
|---|---|
| Gene Synonyms | GPBB |
| Gene Full Name | glycogen phosphorylase Bprovided by HGNC Primary source HGNC:HGNC:9723 |
| Gene Summary | The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 105 kDa |
WB
Western blot analysis of various lysates using PYGB pAb at 1:1000 dilution.
WB
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg PYGB antibody. Western blot was performed from the immunoprecipitate using PYGB antibody at a dilution of 1:1000.
ICC/IF
Immunofluorescence analysis of HepG2 cells using PYGB pAb at dilution of 1:100 (40x lens).| Application Notes | WB:1:500-1:2000 IP:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
