WB
Western blot analysis of VEGF expression in rat spleen tissue sample.WB
Western blot analysis of extracts from Hybridoma cells, using VEGF Antibody. The lane on the left was treated with blocking peptide.WB
Western blot analysis of extracts from various samples, using VEGF Antibody. Lane 1: Pc12, treated with blocking peptide; Lane 2: Pc12; Lane 3: Hela cells. Observed bands: 25 kDa.IHC
VEGFA Antibody at 1/100 staining human colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.IHC
VEGFA Antibody at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | VEGFA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human VEGFA, corresponding to a region within the internal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VEGFA |
|---|---|
| Gene Synonyms | VPF VEGF MVCD1 L-VEGF |
| Gene Full Name | vascular endothelial growth factor A |
| Gene Summary | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020] |
| Alternative Names | Folliculostellate cell-derived growth factor Glioma-derived endothelial cell mitogen MGC70609 MVCD1 Vascular endothelial growth factor A vascular endothelial growth factor A121 vascular endothelial growth factor A165 vascular endothelial growth factor Vascular permeability factor VEGF A Vegf VEGF-A VEGF120 Vegfa VEGFA_HUMAN VPF |
| Molecular Weight(MW) | 16-20kDa,25-30kDa,40-45kDa; 27kD(Calculated). |
| Cellular Localization | Secreted. |
WB
Western blot analysis of VEGF expression in rat spleen tissue sample.
WB
Western blot analysis of extracts from Hybridoma cells, using VEGF Antibody. The lane on the left was treated with blocking peptide.
WB
Western blot analysis of extracts from various samples, using VEGF Antibody. Lane 1: Pc12, treated with blocking peptide; Lane 2: Pc12; Lane 3: Hela cells. Observed bands: 25 kDa.
IHC
VEGFA Antibody at 1/100 staining human colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
IHC
VEGFA Antibody at 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
