WB
25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 polyclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-2 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-2 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.| Product Name | Bcl-2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human Bcl-2 : 101-160/236 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-5000 IHC:1:200-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BCL2 |
|---|---|
| Gene Synonyms | Bcl-2 PPP1R50 |
| Gene Full Name | BCL2 apoptosis regulator |
| Gene Summary | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | Bcl-2 PPP1R50 BCL2_HUMAN BCL2 BCL2 apoptosis regulator B-cell CLL/lymphoma 2 BCL2 apoptosis regulator protein phosphatase 1 regulatory subunit 50 |
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 polyclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-2 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Bcl-2 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.| Application Notes | WB:1:1000-5000 IHC:1:200-500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
