WB
Western blot analysis of KMT2C / MLL3 on different lysates with Rabbit anti-KMT2C / MLL3 antibody at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane), Lane 2: MCF7 cell lysate (20 µg/Lane), Lane 3: HCT 116 cell lysate (20 µg/Lane), Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane), Exposure time: 1 minute 36 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | KMT2C / MLL3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human KMT2C aa 3,251-3,550 / 4,911. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Kmt2c |
|---|---|
| Gene Full Name | lysine methyltransferase 2C |
| Gene Summary | Predicted to enable histone H3K4 methyltransferase activity and transcription coactivator activity. Involved in response to electrical stimulus. Predicted to be located in cytosol and nucleoplasm. Predicted to be part of MLL3/4 complex. Used to study rheumatoid arthritis. Human ortholog(s) of this gene implicated in Kleefstra syndrome 2; carcinoma (multiple); and stomach cancer. Orthologous to human KMT2C (lysine methyltransferase 2C). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Histone-lysine N-methyltransferase 2C EC:2.1.1.364 Lysine N-methyltransferase 2C Homologous to ALR protein Myeloid/lymphoid or mixed-lineage leukemia protein 3 KMT2C HALR KIAA1506 MLL3 |
| Molecular Weight(MW) | 541 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of KMT2C / MLL3 on different lysates with Rabbit anti-KMT2C / MLL3 antibody at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 µg/Lane), Lane 2: MCF7 cell lysate (20 µg/Lane), Lane 3: HCT 116 cell lysate (20 µg/Lane), Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane), Exposure time: 1 minute 36 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
