WB
WB result of TOP2B antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.IP
IP result of anti-TOP2B (IP:4ug; Detection:1:300) with HepG2 cells lysate 3200ug.IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer using TOP2B antibody at dilution of 1:100 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer using TOP2B antibody at dilution of 1:50 (under 40x lens).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescent analysis of HepG2 cells, using TOP2B antibody at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).| Product Name | TOP2B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:20-1:200 ICC/IF:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TOP2B |
|---|---|
| Gene Synonyms | BILU TOPIIB top2beta |
| Gene Full Name | DNA topoisomerase II beta |
| Gene Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
| Alternative Names | DNA topoisomerase 2 beta TOP2B top2beta TOPIIB |
| Molecular Weight(MW) | 183 kDa |
| Cellular Localization | Nucleus. |
WB
WB result of TOP2B antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.
IP
IP result of anti-TOP2B (IP:4ug; Detection:1:300) with HepG2 cells lysate 3200ug.
IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer using TOP2B antibody at dilution of 1:100 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human cervical cancer using TOP2B antibody at dilution of 1:50 (under 40x lens).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescent analysis of HepG2 cells, using TOP2B antibody at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).| Application Notes | WB:1:500-1:2000 IHC:1:20-1:200 ICC/IF:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
