WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HDAC8 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela, Lane 2: K562 , Lane 3: Rat brain, Lane 4: Mouse brain ,IHC
Human smooth muscle was stained with anti-HDAC8 Rabbit antibody| Product Name | HDAC8 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:500 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HDAC8 |
|---|---|
| Gene Synonyms | HD8 WTS RPD3 CDA07 CDLS5 KDAC8 MRXS6 HDACL1 |
| Gene Full Name | histone deacetylase 8 |
| Gene Summary | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Alternative Names | HDAC8 HDACL1 CDA07 Histone deacetylase 8 HD8 |
| Molecular Weight(MW) | 44 kDa |
| Cellular Localization | Nucleus . Chromosome . Cytoplasm . Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HDAC8 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela, Lane 2: K562 , Lane 3: Rat brain, Lane 4: Mouse brain ,
IHC
Human smooth muscle was stained with anti-HDAC8 Rabbit antibody| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:500 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
