WB
Western blot analysis of lysates from Mouse spleen、Mouse thymus、Rat spleen using CD69 Rabbit PolymAb® at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.IHC
mmunohistochemistry analysis of paraffinembedded Human tonsil tissue using CD69 Rabbit PolymAb® at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC stainingICC/IF
Confocal imaging of paraffin-embedded Human tonsil tissue using CD69 Rabbit PolymAb® (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Product Name | CD69 Rabbit PolymAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2500-1:10000 ICC/IF:1:200-1:800 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD69 |
|---|---|
| Gene Synonyms | AIM EA1 MLR-3 CLEC2C GP32/28 BL-AC/P26 |
| Gene Full Name | CD69 molecule |
| Gene Summary | This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011] |
| Alternative Names | AIM EA1 MLR-3 CLEC2C GP32/28 BL-AC/P26 |
| Molecular Weight(MW) | 25-40kDa |
| Cellular Localization | Membrane, Single-pass type II membrane protein. |
WB
Western blot analysis of lysates from Mouse spleen、Mouse thymus、Rat spleen using CD69 Rabbit PolymAb® at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.
IHC
mmunohistochemistry analysis of paraffinembedded Human tonsil tissue using CD69 Rabbit PolymAb® at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining
ICC/IF
Confocal imaging of paraffin-embedded Human tonsil tissue using CD69 Rabbit PolymAb® (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.| Application Notes | WB:1:2500-1:10000 ICC/IF:1:200-1:800 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
