WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VASP (Phospho Ser239) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3, Lane 2: NIH-3T3 was treated with Forskolin (10μM) for 1 hour, Lane 3: C6, Lane 4: C6 was treated with Forskolin (10μM) for 1 hour.IHC
Human placenta was stained with anti-VASP (Phospho Ser239) Rabbit antibody| Product Name | VASP (Phospho Ser239) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Modification | p-Ser239 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | VASP |
|---|---|
| Gene Full Name | vasodilator stimulated phosphoprotein |
| Gene Summary | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008] |
| Alternative Names | Vasodilator-stimulated phosphoprotein VASP |
| Molecular Weight(MW) | 48kD |
| Cellular Localization | Cytoplasm. Cytoplasm , cytoskeleton. Cell junction , focal adhesion. Cell junction , tight junction . Cell projection , lamellipodium membrane. Cell projection , filopodium membrane. Targeted to stress fibers and focal |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VASP (Phospho Ser239) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3, Lane 2: NIH-3T3 was treated with Forskolin (10μM) for 1 hour, Lane 3: C6, Lane 4: C6 was treated with Forskolin (10μM) for 1 hour.
IHC
Human placenta was stained with anti-VASP (Phospho Ser239) Rabbit antibody| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
