WB
Various lysates were subjected to SDS PAGE followed by western blot with KIAA1715 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using KIAA1715 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit
ICC/IF
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using KIAA1715 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).