WB
Western blot analysis of Dopamine Transporter on different lysates with Rat anti-Dopamine Transporter antibody at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.IF-F
Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not requiredIF-F
Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Dopamine Transporter antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Dopamine Transporter antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Dopamine Transporter Recombinant Antibody - Rat IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Tested Applications | IF-FIHCWB |
| IF-F:1:500 IHC:1:500-1:2000 WB:1:2000 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Slc6a3 |
|---|---|
| Gene Synonyms | DAT Dat1 |
| Gene Full Name | solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 |
| Gene Summary | Enables dopamine binding activity; dopamine:sodium symporter activity; and neurotransmitter transmembrane transporter activity. Involved in dopamine uptake; hyaloid vascular plexus regression; and neurotransmitter uptake. Acts upstream of or within several processes, including dopamine metabolic process; lactation; and prepulse inhibition. Located in axon terminus and cell surface. Is expressed in genitourinary system; nervous system; spleen; and submandibular gland. Used to study Gilles de la Tourette syndrome; attention deficit hyperactivity disorder; and schizophrenia. Human ortholog(s) of this gene implicated in attention deficit hyperactivity disorder; classic dopamine transporter deficiency syndrome; mild cognitive impairment; nicotine dependence; and obesity. Orthologous to human SLC6A3 (solute carrier family 6 member 3). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | DA transporter antibody DAT 1 antibody DAT antibody DAT1 antibody Dopamine transporter 1 antibody Dopamine transporter antibody PKDYS antibody SC6A3_HUMAN antibody SLC6A3 antibody Sodium dependent dopamine transporter antibody DA transporter antibody DAT 1 antibody DAT antibody DAT1 antibody Dopamine transporter 1 antibody Dopamine transporter antibody PKDYS antibody SC6A3_HUMAN antibody SLC6A3 antibody Sodium dependent dopamine transporter antibody Sodium-dependent dopamine transporter antibody Solute carrier family 6 (neurotransmitter transporter dopamine) member 3 antibody Solute carrier family 6 (neurotransmitter transporter) member 3 antibody Solute carrier family 6 member 3 antibody Variable number tandem repeat (VNTR) antibody |
| Molecular Weight(MW) | Predicted band size: 69 kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of Dopamine Transporter on different lysates with Rat anti-Dopamine Transporter antibody at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody at 1/5,000 dilution was used for 1 hour at room temperature.
IF-F
Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
IF-F
Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Dopamine Transporter antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Dopamine Transporter antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | IF-F:1:500 IHC:1:500-1:2000 WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
