WB
Western blot analysis of SV2A on different lysates with Rabbit anti-SV2A antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: NIH/3T3 cell lysate (negative) Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SV2A antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not requiredIP
SV2A was immunoprecipitated from 0.2 mg mouse brain tissue lysate at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brain tissue lysate (input) Lane 2: IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of SV2A in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST| Product Name | SV2A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within N terminal Human SV2A. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:5000 IHC:1:500-1:2000 ICC/IF:1:50-1:100 IP: 1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SV2A |
|---|---|
| Gene Synonyms | SV2 DEE113 SLC22B1 |
| Gene Full Name | synaptic vesicle glycoprotein 2A |
| Gene Summary | The protein encoded by this gene is one of three related synaptic vesicle proteins. The encoded protein may interact with synaptotagmin to enhance low frequency neurotransmission in quiescent neurons. [provided by RefSeq, Jun 2016] |
| Alternative Names | KIAA0736 antibody OTTHUMP00000014065 antibody SV2 antibody Sv2a antibody SV2A_HUMAN antibody Synaptic vesicle glycoprotein 2 antibody Synaptic vesicle glycoprotein 2A antibody Synaptic vesicle protein 2a antibody |
| Molecular Weight(MW) | 83 kDa |
| Cellular Localization | Presynapse, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. |
WB
Western blot analysis of SV2A on different lysates with Rabbit anti-SV2A antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: NIH/3T3 cell lysate (negative) Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-SV2A antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
IP
SV2A was immunoprecipitated from 0.2 mg mouse brain tissue lysate at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: mouse brain tissue lysate (input) Lane 2: IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of SV2A in mouse brain tissue lysate Blocking/Dilution buffer: 5% NFDM/TBST| Application Notes | WB:1:5000 IHC:1:500-1:2000 ICC/IF:1:50-1:100 IP: 1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
