WB
Western blot analysis of LINE-1 ORF1p on different lysates with Rabbit anti-LINE-1 ORF1p antibody at 1/1,000 dilution. Lane 1: F9 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane) Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue with Rabbit anti-LINE-1 ORF1p antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of F9 (positive) and NIH/3T3 (negative) labeling LINE-1 ORF1p with Rabbit anti-LINE-1 ORF1p antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LINE-1 ORF1p antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of NIH/3T3 (left, negative) and F9 (right, positive) cells labeling LINE-1 ORF1p. Cells were fixed and permeabilized. Then stained with the primary antibody ( 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
LINE-1 ORF1p was immunoprecipitated from 0.2 mg F9 cell lysate with at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: F9 cell lysate (input) Lane 2: IP in F9 cell lysate Lane 3: Rabbit IgG instead in F9 cell lysate Blocking/Dilution buffer: primary antibody dilution| Product Name | LINE-1 ORF1p Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse LINE-1 ORF1p aa 1-357. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1000 IHC:1:50 ICC/IF:1:100 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LINE-1 retrotransposable element ORF1 protein antibody L1-ORF1p, LINE retrotransposable element 1 antibody LINE1 retrotransposable element 1 antibody Transposase element L1Md-A101/L1Md-A102/L1Md-A2 antibody Lire1 antibody |
|---|---|
| Alternative Names | LINE-1 retrotransposable element ORF1 protein antibody L1-ORF1p LINE retrotransposable element 1 antibody LINE1 retrotransposable element 1 antibody Transposase element L1Md-A101/L1Md-A102/L1Md-A2 antibody Lire1 antibody |
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Nucleus, nucleolus, Cytoplasm, Cytoplasmic ribonucleoprotein granule, Stress granule. |
WB
Western blot analysis of LINE-1 ORF1p on different lysates with Rabbit anti-LINE-1 ORF1p antibody at 1/1,000 dilution. Lane 1: F9 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane) Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue with Rabbit anti-LINE-1 ORF1p antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of F9 (positive) and NIH/3T3 (negative) labeling LINE-1 ORF1p with Rabbit anti-LINE-1 ORF1p antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LINE-1 ORF1p antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of NIH/3T3 (left, negative) and F9 (right, positive) cells labeling LINE-1 ORF1p. Cells were fixed and permeabilized. Then stained with the primary antibody ( 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
LINE-1 ORF1p was immunoprecipitated from 0.2 mg F9 cell lysate with at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: F9 cell lysate (input) Lane 2: IP in F9 cell lysate Lane 3: Rabbit IgG instead in F9 cell lysate Blocking/Dilution buffer: primary antibody dilution| Application Notes | WB:1:1000 IHC:1:50 ICC/IF:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
