WB
Various lysates were subjected to SDS PAGE followed by western blot with Cyclin A2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Cyclin A2 antibody at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using Cyclin A2 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).| Product Name | Cyclin A2 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Excitation/Emission maxima wavelengths: 588 nm / 604 nm |
| Immunogen | Recombinant human Cyclin A2 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:50-1:500 WB:1:2000-1:10000 IHC:1:1000-1:4000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNA2 |
|---|---|
| Gene Synonyms | CCN1 CCNA |
| Gene Full Name | cyclin A2 |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016] |
| Alternative Names | CCN1 CCNA CCNA2 Cyclin A Cyclin A2 cyclinA2 |
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Cyclin A2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Cyclin A2 antibody at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using Cyclin A2 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | ICC/IF:1:50-1:500 WB:1:2000-1:10000 IHC:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
