WB
Sample:Testis (Mouse) Lysate at 40 ugPrimary: Anti-FSH receptor at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kDObserved band size: 78 kDIHC
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSH receptor) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.IHC
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-FSH receptor Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB stainingFC
Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-FSH receptor antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0895R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | CDKN1A/P21 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human FSHR : 201-300/695 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHC-FIHC-PWB |
| WB:1:500-2000 IHC-P:1:100-500 IHC-F:1:100-500 FC:1μg/Test |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FSHR |
|---|---|
| Gene Synonyms | LGR1 ODG1 FSHR1 FSHRO |
| Gene Full Name | follicle stimulating hormone receptor |
| Gene Summary | The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| Alternative Names | FSHR1 FSHRO LGR1 ODG1 FSHRE FSHR_BOVIN FSHR FSH-R Follitropin receptor FSHR_HUMAN FSHR_MOUSE FSHR_PIG FSHR_RAT FSHR_SHEEP follicle stimulating hormone receptor |
| Molecular Weight(MW) | 78 kDa |
| Cellular Localization | Cell membrane,Membrane |
WB
Sample:Testis (Mouse) Lysate at 40 ugPrimary: Anti-FSH receptor at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kDObserved band size: 78 kD
IHC
Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSH receptor) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
IHC
Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-FSH receptor Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
FC
Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-FSH receptor antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0895R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-2000 IHC-P:1:100-500 IHC-F:1:100-500 FC:1μg/Test |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
