WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Phospho-AMPK alpha 1 (S485) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: C6 was treated with Oligomycin(0.5μM) for 30 minutes Lane 3: HEK293 Lane 4: HEK293 was treated with Oligomycin(0.5μM) for 30 minutes Lane 5: C2C12 Lane 6: C2C12 was treated with Oligomycin(0.5μM) for 30 minutes Predicted band size: 62kDa Observed band size: 62kDa| Product Name | Phospho-AMPK alpha 1 (S485) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser485 of Human AMPK alpha 1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2,000-1:10,000 ICC/IF:1:200-1:1,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKAA1 |
|---|---|
| Gene Synonyms | AMPK AMPKa1 AMPK alpha 1 |
| Gene Full Name | protein kinase AMP-activated catalytic subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Alternative Names | 5 AMP activated protein kinase alpha 1catalytic subunit antibody 5 AMP activated protein kinase catalytic alpha 1 chain antibody 5' AMP activated protein kinase catalytic subunit alpha 1 antibody 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody AAPK1 antibody AAPK1_HUMAN antibody ACACA kinase antibody acetyl CoA carboxylase kinase antibody AI194361 antibody AI450832 antibody 5 AMP activated protein kinase alpha 1catalytic subunit antibody 5 AMP activated protein kinase catalytic alpha 1 chain antibody 5' AMP activated protein kinase catalytic subunit alpha 1 antibody 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody AAPK1 antibody AAPK1_HUMAN antibody ACACA kinase antibody acetyl CoA carboxylase kinase antibody AI194361 antibody AI450832 antibody AL024255 antibody AMP -activate kinase alpha 1 subunit antibody AMP-activated protein kinase catalytic alpha -1 antibody AMPK 1 antibody AMPK alpha 1 antibody AMPK alpha 1 chain antibody AMPK antibody AMPK subunit alpha-1 antibody AMPK1 antibody AMPKa1 antibody AMPKalpha1 antibody C130083N04Rik antibody cb116 antibody EC 2.7.11.1 antibody HMG CoA reductase kinase antibody HMGCR kinase antibody hormone sensitive lipase kinase antibody Hydroxymethylglutaryl CoA reductase kinase antibody im:7154392 antibody kinase AMPK alpha1 antibody MGC33776 antibody MGC57364 antibody OTTHUMP00000161795 antibody OTTHUMP00000161796 antibody PRKAA 1 antibody PRKAA1 antibody Protein kinase AMP activated alpha 1 catalytic subunit antibody SNF1-like protein AMPK antibody SNF1A antibody Tau protein kinase PRKAA1 antibody wu:fa94c10 antibody |
| Molecular Weight(MW) | 62kDa |
| Cellular Localization | Cytoplasm,Endosome,Nucleus |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Phospho-AMPK alpha 1 (S485) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: C6 was treated with Oligomycin(0.5μM) for 30 minutes Lane 3: HEK293 Lane 4: HEK293 was treated with Oligomycin(0.5μM) for 30 minutes Lane 5: C2C12 Lane 6: C2C12 was treated with Oligomycin(0.5μM) for 30 minutes Predicted band size: 62kDa Observed band size: 62kDa| Application Notes | WB:1:2,000-1:10,000 ICC/IF:1:200-1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15℃ to -25℃/1 year(Do not lower than -25℃) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
