WB
Western blot analysis of GINS1 using anti-GINS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Hepa1/6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GINS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GINS1 at approximately 23 kDa. The expected band size for GINS1 is at 23 kDa.ICC/IF
ICC/IF analysis of GINS1 using anti-GINS1 antibody and anti-Alpha Tubulin antibody. GINS1 was detected in an immunocytochemical section of MG63 cells. The section was incubated with rabbit anti-GINS1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of A549 cells using anti-GINS1 antibody. Overlay histogram showing A549 cells stained with (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GINS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-GINS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GINS1 recombinant protein (Position: M1-S196). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFWB |
| WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 ELISA:1:100-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GINS1 |
|---|---|
| Gene Synonyms | PSF1 IMD55 |
| Gene Full Name | GINS complex subunit 1 |
| Gene Summary | The yeast heterotetrameric GINS complex is made up of Sld5 (GINS4; MIM 610611), Psf1, Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]).[supplied by OMIM, Mar 2008] |
| Alternative Names | PSF1 KIAA0186 GINS complex subunit 1 |
| Molecular Weight(MW) | 23 kDa |
| Cellular Localization | Chromosome,Nucleus |
WB
Western blot analysis of GINS1 using anti-GINS1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Hepa1/6 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GINS1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GINS1 at approximately 23 kDa. The expected band size for GINS1 is at 23 kDa.
ICC/IF
ICC/IF analysis of GINS1 using anti-GINS1 antibody and anti-Alpha Tubulin antibody. GINS1 was detected in an immunocytochemical section of MG63 cells. The section was incubated with rabbit anti-GINS1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of A549 cells using anti-GINS1 antibody. Overlay histogram showing A549 cells stained with (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GINS1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 ICC/IF:1:50-400 FC:1:50-200 ELISA:1:100-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
