WB
Various lysates were subjected to SDS PAGE followed by western blot with AKAP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using AKAP1 Antibody at dilution of 1:50 (under 40x lens).IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using AKAP1 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-AKAP1 (IP:4ug; Detection:1:2000) with PC-3 cells lysate 1800ug.ICC/IF
Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).| Product Name | AKAP1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human AKAP1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:50-1:500 ICC/IF:1:10-1:100 WB:1:1000-1:4000 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKAP1 |
|---|---|
| Gene Synonyms | AKAP PRKA1 AKAP84 TDRD17 AKAP121 AKAP149 D-AKAP1 PPP1R43 SAKAP84 |
| Gene Full Name | A-kinase anchoring protein 1 |
| Gene Summary | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008] |
| Alternative Names | AKAP AKAP 1 A-kinase anchor protein 1 mitochondrial A-kinase anchor protein 149 kDa DAKAP1 |
| Molecular Weight(MW) | 149 kDa |
| Cellular Localization | Membrane,Mitochondrion,Mitochondrion outer membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with AKAP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using AKAP1 Antibody at dilution of 1:50 (under 40x lens).
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using AKAP1 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-AKAP1 (IP:4ug; Detection:1:2000) with PC-3 cells lysate 1800ug.
ICC/IF
Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).| Application Notes | IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:50-1:500 ICC/IF:1:10-1:100 WB:1:1000-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
