WB
Western blot analysis of various lysates using TNFR1/TNFRSF1A Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:10s.IHC
Immunohistochemistry analysis of paraffin-embedded Rat spleen using TNFR1/TNFRSF1A Rabbit pAb at dilution of 1:100 (40xlens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC stainingICC/IF
Immunofiluorescence analysis of BALB-3T3 cells using TNFR1/TNFRSF1A Rabbit pAb at dilution of 1100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPl for nuclear staining.| Product Name | TNFR1/TNFRSF1A Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:500 - 1:5000 ICC/IF:1:50 - 1:200 IHC:1:50 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF1A |
|---|---|
| Gene Synonyms | FPF p55 p60 TBP1 TNF-R TNFAR TNFR1 p55-R CD120a TNFR55 TNFR60 TNF-R-I TNF-R55 |
| Gene Full Name | TNF receptor superfamily member 1A |
| Gene Summary | This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients. [provided by RefSeq, Sep 2016] |
| Alternative Names | FPF p55 p60 TBP1 TNF-R TNFAR TNFR1 p55-R CD120a TNFR55 TNFR60 TNF-R-I TNF-R55 TNFR1/TNFRSF1A |
| Molecular Weight(MW) | 50kDa(Observed band size:55kDa) |
| Cellular Localization | Cell membrane,Golgi apparatus,Membrane,Secreted |
WB
Western blot analysis of various lysates using TNFR1/TNFRSF1A Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:10s.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat spleen using TNFR1/TNFRSF1A Rabbit pAb at dilution of 1:100 (40xlens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining
ICC/IF
Immunofiluorescence analysis of BALB-3T3 cells using TNFR1/TNFRSF1A Rabbit pAb at dilution of 1100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPl for nuclear staining.| Application Notes | WB:1:500 - 1:5000 ICC/IF:1:50 - 1:200 IHC:1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
