WB
Western blot analysis of GABARAPL1-Specific using anti-GABARAPL1-Specific antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GABARAPL1-Specific antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GABARAPL1-Specific at approximately 19 kDa. The expected band size for GABARAPL1-Specific is at 14 kDa.IHC
IHC analysis of GABARAPL1-Specific using anti-GABARAPL1-Specific antibody. GABARAPL1-Specific was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-GABARAPL1-Specific Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Anti-GABARAPL1-Specific Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GABARAPL1-Specific recombinant protein (Position: M1-E112). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFIHCIPWB |
| WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1-3 μg/1x106 cells IP:1:50 ELISA:1:100-1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABARAPL1 |
|---|---|
| Gene Synonyms | ATG8 GEC1 APG8L ATG8B ATG8L APG8-LIKE |
| Gene Full Name | GABA type A receptor associated protein like 1 |
| Gene Summary | Enables Tat protein binding activity; phospholipid binding activity; and ubiquitin protein ligase binding activity. Predicted to be involved in cellular response to nitrogen starvation and macroautophagy. Located in several cellular components, including autophagosome; ciliary basal body; and mitochondrion. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | APG8 LIKE APG8L ATG8 ATG8L GABARAPL1 |
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Cytoplasm,Cytoplasmic vesicle,Cytoskeleton,Endoplasmic reticulum,Golgi apparatus |
WB
Western blot analysis of GABARAPL1-Specific using anti-GABARAPL1-Specific antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GABARAPL1-Specific antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GABARAPL1-Specific at approximately 19 kDa. The expected band size for GABARAPL1-Specific is at 14 kDa.
IHC
IHC analysis of GABARAPL1-Specific using anti-GABARAPL1-Specific antibody. GABARAPL1-Specific was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-GABARAPL1-Specific Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-2000 IHC:1:50-400 ICC/IF:1:50-400 FC:1-3 μg/1x106 cells IP:1:50 ELISA:1:100-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
