WB
Western blot analysis of LGALS3BP using anti-LGALS3BP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO 320 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LGALS3BP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LGALS3BP at approximately 70-90 kDa. The expected band size for LGALS3BP is at 65 kDa.ICC/IF
ICC/IF analysis of LGALS3BP using anti-LGALS3BP antibody. LGALS3BP was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-LGALS3BP Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-LGALS3BP antibody. Overlay histogram showing HepG2 cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LGALS3BP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Anti-LGALS3BP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human LGALS3BP recombinant protein (Position: H115-K508). Human LGALS3BP shares 67.9% and 66.2% amino acid (aa) sequence identity with mouse and rat LGALS3BP, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFCICC/IFWB |
| WB:1:500-2000 ELISA:1:100-1000 ICC/IF:1:50-400 FC:1:50-200 |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STMN1 |
|---|---|
| Gene Synonyms | Lag SMN OP18 PP17 PP19 PR22 LAP18 C1orf215 |
| Gene Full Name | stathmin 1 |
| Gene Summary | This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
| Alternative Names | 90K BTBD17B Galectin 3 binding protein LGALS3BP M2BP Mac 2 binding protein MAC 2 BP MAC2BP Tumor associated antigen 90K |
| Molecular Weight(MW) | 65 kDa |
| Cellular Localization | Extracellular matrix,Secreted |
WB
Western blot analysis of LGALS3BP using anti-LGALS3BP antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO 320 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LGALS3BP antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for LGALS3BP at approximately 70-90 kDa. The expected band size for LGALS3BP is at 65 kDa.
ICC/IF
ICC/IF analysis of LGALS3BP using anti-LGALS3BP antibody. LGALS3BP was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-LGALS3BP Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-LGALS3BP antibody. Overlay histogram showing HepG2 cells stained with (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LGALS3BP Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-2000 ELISA:1:100-1000 ICC/IF:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
