WB
Various lysates were subjected to SDS PAGE followed by western blot with Recoverin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using Recoverin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Recoverin antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | Recoverin Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Recoverin protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:500-1:3000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:50-1:500 IF-P:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | RGR |
|---|---|
| Gene Synonyms | RP44 |
| Gene Full Name | retinal G protein coupled receptor |
| Gene Summary | This gene encodes a putative retinal G-protein coupled receptor. The gene is a member of the opsin subfamily of the 7 transmembrane, G-protein coupled receptor 1 family. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. The protein acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The protein is exclusively expressed in tissue adjacent to retinal photoreceptor cells, the retinal pigment epithelium and Mueller cells. This gene may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | RCVRN Cancer associated retinopathy protein Cancer-associated retinopathy protein Protein CAR RCV1 |
| Molecular Weight(MW) | 24 kDa(Observed band size:23 kDa) |
| Cellular Localization | Cell projection,Membrane |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Recoverin antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using Recoverin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Recoverin antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:3000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:50-1:500 IF-P:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
