WB
Western blot analysis of Phospho-Axl on different lysates with Rabbit anti-Phospho-Axl antibody at 1/5,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate Lane 3: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-Axl (Y702) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr702 of human Axl. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5,000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AXL |
|---|---|
| Gene Synonyms | ARK UFO AXL3 JTK11 Tyro7 |
| Gene Full Name | AXL receptor tyrosine kinase |
| Gene Summary | The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. The encoded protein acts as a host cell receptor for multiple viruses, including Marburg, Ebola and Lassa viruses and is a candidate receptor for the SARS-CoV2 virus. [provided by RefSeq, Sep 2021] |
| Alternative Names | Adhesion related kinase antibody AI323647 antibody Ark antibody Axl antibody AXL oncogene antibody AXL receptor tyrosine kinase antibody AXL transforming gene antibody AXL transforming sequence/gene antibody EC 2.7.10.1 antibody JTK11 antibody Adhesion related kinase antibody AI323647 antibody Ark antibody Axl antibody AXL oncogene antibody AXL receptor tyrosine kinase antibody AXL transforming gene antibody AXL transforming sequence/gene antibody EC 2.7.10.1 antibody JTK11 antibody Oncogene AXL antibody Tyro7 antibody Tyrosine protein kinase receptor UFO antibody Tyrosine-protein kinase receptor UFO antibody UFO antibody UFO_HUMAN antibody |
| Molecular Weight(MW) | 98 kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of Phospho-Axl on different lysates with Rabbit anti-Phospho-Axl antibody at 1/5,000 dilution. Lane 1: NCI-H1299 cell lysate Lane 2: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate Lane 3: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:5,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
