WB
Western blot analysis of anti-OSBPL8 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OSBPL8 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for OSBPL8 at approximately 120 kDa. The expected band size for OSBPL8 is at 101 kDa.ICC/IF
ICC/IF analysis of OSBPL8 using anti-OSBPL8 antibody. OSBPL8 was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-OSBPL8 antibody. Overlay histogram showing HepG2 cells stained with Blue line. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSBPL8 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-OSBPL8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human OSBPL8 recombinant protein (Position: D33-K889). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OSBPL8 |
|---|---|
| Gene Synonyms | ORP8 MST120 OSBP10 MSTP120 |
| Gene Full Name | oxysterol binding protein like 8 |
| Gene Summary | This gene encodes a member of a family of proteins containing an N-terminal pleckstrin homology domain and a highly conserved C-terminal oxysterol-binding protein-like sterol-binding domain. It binds mutliple lipid-containing molecules, including phosphatidylserine, phosphatidylinositol 4-phosphate (PI4P) and oxysterol, and promotes their exchange between the endoplasmic reticulum and the plasma membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016] |
| Alternative Names | OSBPL8 KIAA1451 ORP8 OSBP10 Oxysterol-binding protein-related protein 8 ORP-8 OSBP-related protein 8 |
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane,Nucleus |
WB
Western blot analysis of anti-OSBPL8 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OSBPL8 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for OSBPL8 at approximately 120 kDa. The expected band size for OSBPL8 is at 101 kDa.
ICC/IF
ICC/IF analysis of OSBPL8 using anti-OSBPL8 antibody. OSBPL8 was detected in an immunocytochemical section of Hela cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-OSBPL8 antibody. Overlay histogram showing HepG2 cells stained with Blue line. To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSBPL8 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
