WB
Various lysates were subjected to SDS PAGE followed by western blot with (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (CBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Product Name | CBLL1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human CBLL1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWB |
| WB:1:1000-1:6000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|
| Species Reactivity | HumanMouse |
| Purification | Affinity purified |
| Gene Symbol | CBLL1 |
|---|---|
| Gene Synonyms | HAKAI RNF188 |
| Gene Full Name | Cbl proto-oncogene like 1 |
| Gene Summary | This gene encodes an E3 ubiquitin-ligase for the E-cadherin complex and mediates its ubiquitination, endocytosis, and degradation in the lysosomes. The encoded protein contains a RING-finger domain and is also thought to have a role in control of cell proliferation. A related pseudogene has been identified on chromosome X. Alternative splicing results in a non-coding transcript variant. [provided by RefSeq, Aug 2011] |
| Alternative Names | HAKAI EC:2.3.2.27 E3 ubiquitin-protein ligase Hakai c-Cbl-like protein 1 Casitas B-lineage lymphoma-transforming sequence-like protein 1 |
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (CBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:1000-1:6000 IHC:1:50-1:500 ICC/IF:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
