WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDK6 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat Lane 3: K562IHC
Human tonsil was stained with Anti-CDK6 rabbit antibodyICC/IF
Immunofluorescence analysis of HEK293. Picture A: CDK6 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Product Name | CDK6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human TMSB4X protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-5000 IHC:1:200-1000ICC/IF:1:200-1000 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK6 |
|---|---|
| Gene Synonyms | MCPH12 PLSTIRE |
| Gene Full Name | cyclin dependent kinase 6 |
| Gene Summary | The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017] |
| Alternative Names | Cyclin-dependent kinase 6 Cell division protein kinase 6 Serine/threonine-protein kinase PLSTIRE |
| Molecular Weight(MW) | 37kD |
| Cellular Localization | Nuclear |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDK6 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Jurkat Lane 3: K562
IHC
Human tonsil was stained with Anti-CDK6 rabbit antibody
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: CDK6 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B| Application Notes | WB:1:1000-5000 IHC:1:200-1000ICC/IF:1:200-1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
