WB
Western blot analysis of Phospho-Beta Catenin (S675) on different lysates with Rabbit anti-Phospho-Beta Catenin (S675) antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lane 4: Rat brain tissue lysate Lane 5: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 1 minute; Lane 4-5: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724534, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 85 kDa Observed band size: 85 kDaIHC
Application: Immunohistochemistry Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724534, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature.IP
Immunoprecipitation (IP) Phospho-Beta Catenin (S675) was immunoprecipitated in 0.2 mg mouse brain tissue lysate with HA724534 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724534 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA724534 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA724534 in mouse brain tissue lysate Exposure time: 25 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724534, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 85 kDa Observed band size: 85 kDa| Product Name | Phospho-Beta Catenin (S675) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser675 of Human Beta catenin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:5,000 IHC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CTNNB1 |
|---|---|
| Gene Synonyms | EVR7 CTNNB MRD19 NEDSDV armadillo |
| Gene Full Name | catenin beta 1 |
| Gene Summary | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
| Alternative Names | b-catenin antibody Beta catenin antibody Beta-catenin antibody Cadherin associated protein antibody Catenin (cadherin associated protein) beta 1 88kDa antibody Catenin beta 1 antibody Catenin beta-1 antibody CATNB antibody CHBCAT antibody CTNB1_HUMAN antibody b-catenin antibody Beta catenin antibody Beta-catenin antibody Cadherin associated protein antibody Catenin (cadherin associated protein) beta 1 88kDa antibody Catenin beta 1 antibody Catenin beta-1 antibody CATNB antibody CHBCAT antibody CTNB1_HUMAN antibody CTNNB antibody CTNNB1 antibody DKFZp686D02253 antibody FLJ25606 antibody FLJ37923 antibody OTTHUMP00000162082 antibody OTTHUMP00000165222 antibody OTTHUMP00000165223 antibody OTTHUMP00000209288 antibody OTTHUMP00000209289 antibody |
| Molecular Weight(MW) | 85 kDa |
WB
Western blot analysis of Phospho-Beta Catenin (S675) on different lysates with Rabbit anti-Phospho-Beta Catenin (S675) antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lane 4: Rat brain tissue lysate Lane 5: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-3: 1 minute; Lane 4-5: 2 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA724534, 1/5,000 in primary antibody dilution buffer, overnight at 4 °C Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 85 kDa Observed band size: 85 kDa
IHC
Application: Immunohistochemistry Species: Human Tissue: Colon Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA724534, 1/4,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature.
IP
Immunoprecipitation (IP) Phospho-Beta Catenin (S675) was immunoprecipitated in 0.2 mg mouse brain tissue lysate with HA724534 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724534 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA724534 IP in mouse brain tissue lysate Lane 3: Rabbit IgG instead of HA724534 in mouse brain tissue lysate Exposure time: 25 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary dilution: HA724534, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature Predicted band size: 85 kDa Observed band size: 85 kDa| Application Notes | WB:1:5,000 IHC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
