WB
Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody at 1/1,000 dilution. Lane 1: Mouse ovary tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat ovary tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40-45 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human ovary granular cell tumor tissue with Rabbit anti-Inhibin alpha antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Inhibin alpha Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Inhibin alpha aa 233-366. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:500-1:1,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | INHA |
|---|---|
| Gene Full Name | inhibin subunit alpha |
| Gene Summary | This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients. [provided by RefSeq, Aug 2016] |
| Alternative Names | A inhibin subunit antibody A inhibin subunit precursor antibody AW55578 antibody IHA antibody inhA antibody INHA_HUMAN antibody Inhibin alpha chain antibody Inhibin alpha chain precursor antibody Inhibin alpha subunit antibody |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Secreted |
WB
Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody at 1/1,000 dilution. Lane 1: Mouse ovary tissue lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat ovary tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40-45 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human ovary granular cell tumor tissue with Rabbit anti-Inhibin alpha antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:500-1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
