WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Acetyl Lysine antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela treated overnight with TSA Lane 3: NIH-3T3 Lane 4: NIH-3T3 treated overnight with TSAIHC
Rat liver was stained with anti-Acetyl Lysine Rabbit antibody| Product Name | Acetyl Lysine Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:500-1:2000 IHC:1:1000-1:2000 IF:1:200-1:1000 ELISA:1:5000-1:20000 IP:1:50-1:200 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EP300 |
|---|---|
| Gene Synonyms | p300 KAT3B MKHK2 RSTS2 |
| Gene Full Name | EP300 lysine acetyltransferase |
| Gene Summary | This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008] |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Acetyl Lysine antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela treated overnight with TSA Lane 3: NIH-3T3 Lane 4: NIH-3T3 treated overnight with TSA
IHC
Rat liver was stained with anti-Acetyl Lysine Rabbit antibody| Application Notes | WB:1:500-1:2000 IHC:1:1000-1:2000 IF:1:200-1:1000 ELISA:1:5000-1:20000 IP:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
