WB
Western blot analysis of Phospho-MYPT1 (T696) on different lysates with Rabbit anti-Phospho-MYPT1 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM Y-27632 for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 140 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-MYPT1 (T696) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr696 of Human MYPT1. |
| Modification | p-T696 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPP1R12A |
|---|---|
| Gene Synonyms | MBS GUBS M130 MYPT1 |
| Gene Full Name | protein phosphatase 1 regulatory subunit 12A |
| Gene Summary | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| Alternative Names | M130 antibody MBS antibody Myosin binding subunit antibody Myosin phosphatase target subunit 1 antibody Myosin phosphatase targeting subunit 1 antibody Myosin phosphatase-targeting subunit 1 antibody MYPT1 antibody MYPT1_HUMAN antibody PPP1R12A antibody Protein phosphatase 1 regulatory inhibitor subunit 12A antibody M130 antibody MBS antibody Myosin binding subunit antibody Myosin phosphatase target subunit 1 antibody Myosin phosphatase targeting subunit 1 antibody Myosin phosphatase-targeting subunit 1 antibody MYPT1 antibody MYPT1_HUMAN antibody PPP1R12A antibody Protein phosphatase 1 regulatory inhibitor subunit 12A antibody Protein phosphatase 1 regulatory subunit 12A antibody Protein phosphatase 1 regulatory (inhibitor) subunit 12A antibody Protein phosphatase myosin binding subunit antibody Protein phosphatase myosin-binding subunit antibody |
| Molecular Weight(MW) | 115 kDa(Observed band size: 140 kDa) |
| Cellular Localization | Cytoplasm,Cytoskeleton |
WB
Western blot analysis of Phospho-MYPT1 (T696) on different lysates with Rabbit anti-Phospho-MYPT1 antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM Y-27632 for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 115 kDa Observed band size: 140 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4). |
