WB
Western blot analysis of lysates from A-431 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb at 1:1000 dilution. A 431 cells were treated with EGF (200 ng/ml) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:45s.IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 RabbitmAb at a dllution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.IP
Immunoprecipitation of Phospho-CDK1-T161 in 500 ug extracts from HeLa cells treated with UV(100 mj, 4h) using 2 g Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb. Western blot analysis was performed using Phospho-CDK1-T161 Rabbit mAb at 1:1000 dilution.IF
Confocal imaging of paraffin-embedded Human tesits tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb followed by a further incubation with Cy3 Goat Anti- Rabbit lgG (H+L) (Red). DAPIwas used for nudearstaining (Blue) Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Product Name | Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISAIFIHCIPWB |
| WB:1:500 - 1:1000 IHC:1:100 - 1:500 IF:1:50 - 1:200 IP:0.5μg-4μg antibody for 400μg-600μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK1 |
|---|---|
| Gene Synonyms | CDC2 CDC28A P34CDC2 |
| Gene Full Name | cyclin dependent kinase 1 |
| Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2023] |
| Alternative Names | CDC2 CDC28A P34CDC2 CDK1 |
| Molecular Weight(MW) | 34kDa |
| Cellular Localization | Cytoplasm,Cytoskeleton,Mitochondrion,Nucleus |
WB
Western blot analysis of lysates from A-431 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb at 1:1000 dilution. A 431 cells were treated with EGF (200 ng/ml) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time:45s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 RabbitmAb at a dllution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation of Phospho-CDK1-T161 in 500 ug extracts from HeLa cells treated with UV(100 mj, 4h) using 2 g Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb. Western blot analysis was performed using Phospho-CDK1-T161 Rabbit mAb at 1:1000 dilution.
IF
Confocal imaging of paraffin-embedded Human tesits tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb followed by a further incubation with Cy3 Goat Anti- Rabbit lgG (H+L) (Red). DAPIwas used for nudearstaining (Blue) Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:500 - 1:1000 IHC:1:100 - 1:500 IF:1:50 - 1:200 IP:0.5μg-4μg antibody for 400μg-600μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
