WB
Western blot analysis of COUP TF1 on different lysates with Rabbit anti-COUP TF1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 8 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-COUP TF1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | COUP TF1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human COUP TF1 aa 1-50 / 423. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:1,000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR2F1 |
|---|---|
| Gene Synonyms | EAR3 BBOAS EAR-3 SVP44 BBSOAS ERBAL3 COUPTF1 TFCOUP1 COUP-TFI TCFCOUP1 |
| Gene Full Name | nuclear receptor subfamily 2 group F member 1 |
| Gene Summary | The protein encoded by this gene is a nuclear hormone receptor and transcriptional regulator. The encoded protein acts as a homodimer and binds to 5'-AGGTCA-3' repeats. Defects in this gene are a cause of Bosch-Boonstra optic atrophy syndrome (BBOAS). [provided by RefSeq, Apr 2014] |
| Alternative Names | Chicken ovalbumin upstream promoter 1 antibody COT1_HUMAN antibody COUP transcription factor 1 antibody COUP transcription factor I antibody COUP-TF I antibody COUP-TF1 antibody EAR-3 antibody EAR3 antibody ERBAL3 antibody NR2F1 antibody Chicken ovalbumin upstream promoter 1 antibody COT1_HUMAN antibody COUP transcription factor 1 antibody COUP transcription factor I antibody COUP-TF I antibody COUP-TF1 antibody EAR-3 antibody EAR3 antibody ERBAL3 antibody NR2F1 antibody NR2F2 antibody Nuclear receptor subfamily 2 group F member 1 antibody SVP44 antibody TCFCOUP1 antibody TFCOUP1 antibody Transcription factor COUP 1 antibody V ERBA related protein EAR 3 antibody V-erbA-related protein 3 antibody |
| Molecular Weight(MW) | Predicted band size: 46 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of COUP TF1 on different lysates with Rabbit anti-COUP TF1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 8 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-COUP TF1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
