WB
Western blot analysis of Slc12a5 / KCC2 on different lysates with Mouse anti-Slc12a5 / KCC2 antibody at 1/5,000 dilution. Lane 1: Mouse hippocampus tissue lysate (no heat) Lane 2: Mouse brain tissue lysate (no heat) Lane 3: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not requiredIHC
Application: IHC-Fr Species: Rat Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not requiredIHC
Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required| Product Name | Slc12a5 / KCC2 Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Rat Slc12a5 / KCC2 aa 932-1043. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FWB |
| WB:1:5000 IHC:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Aim2 |
|---|---|
| Gene Synonyms | Gm1313 Ifi210 |
| Gene Full Name | absent in melanoma 2 |
| Gene Summary | Enables several functions, including identical protein binding activity; pattern recognition receptor activity; and signaling adaptor activity. Involved in several processes, including AIM2 inflammasome complex assembly; negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; and regulation of defense response. Acts upstream of or within cellular response to interferon-beta; positive regulation of inflammatory response; and protein maturation. Part of AIM2 inflammasome complex. Is active in cytoplasm; nucleus; and site of double-strand break. Is expressed in embryo. Orthologous to human AIM2 (absent in melanoma 2). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | Electroneutral potassium chloride cotransporter 2 antibody Electroneutral potassium-chloride cotransporter 2 antibody Erythroid K Cl cotransporter 2 antibody Furosemide sensitive K Cl cotransporter antibody hKCC2 antibody K-Cl cotransporter 2 antibody KCC 2 antibody KCC2 antibody KIAA1176 antibody Neuronal K Cl cotransporter antibody Electroneutral potassium chloride cotransporter 2 antibody Electroneutral potassium-chloride cotransporter 2 antibody Erythroid K Cl cotransporter 2 antibody Furosemide sensitive K Cl cotransporter antibody hKCC2 antibody K-Cl cotransporter 2 antibody KCC 2 antibody KCC2 antibody KIAA1176 antibody Neuronal K Cl cotransporter antibody Neuronal K-Cl cotransporter antibody Potassium Chloride Cotransporter antibody Potassium chloride transporter 5 antibody rKCC2 antibody S12A5 antibody S12A5_HUMAN antibody SLC12A5 antibody Solute carrier family 12 (potassium chloride transporter) member 5 antibody Solute carrier family 12 member 5 antibody |
| Molecular Weight(MW) | 126 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Cell membrane, Cell projection, dendrite. |
WB
Western blot analysis of Slc12a5 / KCC2 on different lysates with Mouse anti-Slc12a5 / KCC2 antibody at 1/5,000 dilution. Lane 1: Mouse hippocampus tissue lysate (no heat) Lane 2: Mouse brain tissue lysate (no heat) Lane 3: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
IHC
Application: IHC-Fr Species: Rat Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
IHC
Application: IHC-Fr Species: Mouse Site: cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required| Application Notes | WB:1:5000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
