IP
c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells. Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins.| Product Name | anti-Myc tag Antibody Conjugated Agarose Affinity Gel |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide |
| Conjugation | Agarose |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IP |
| capacity:≥0.6 mg/mL, gel binding capacity |
|
| Species Reactivity | Tag |
| Purification | Affinity purified |
| Gene Symbol | Myc |
|---|---|
| Gene Summary | The human c-Myc proto-oncogene belongs to MYC oncogene family and is the human cellular homolog of the avian v-Myc gene found in several leukemogenic retroviruses. c-Myc gene is localized on human chromosome 8q24.21. The two major domains of include the N-terminal box regions and the C-terminal basic-region with helix-loop-helix/leucine-zipper motif. |
IP
c-Myc proto-oncogene increased expression is observed in a variety of human tumors. Amplification of Myc gene locus is observed in angiosarcomas and translocation is implicated in Burkitt′s lymphoma. c-Myc is a master regulator and targets a wide variety of metabolic events. An epitope located within amino acid residues 410-419 (EQKLISEEDL) of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tagged fusion proteins. Anti-c-Myc agarose is useful in the purification of expressed c-Myc tagged fusion proteins in bacterial lysates or transfected cells. Anti-c-Myc recognizes an epitope located on c-Myc tagged fusion proteins.| Application Notes | capacity:≥0.6 mg/mL, gel binding capacity |
|---|
| Form | suspension |
|---|---|
| Storage Instructions | For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze. |
| Storage Buffer | Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide |
