| Product Name | anti-His tag Antibody Conjugated Agarose Affinity Gel |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide |
| Conjugation | Agarose |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IP |
| capacity:≥0.6 mg/mL, gel binding capacity |
|
| Species Reactivity | Tag |
| Purification | Affinity purified |
| Gene Symbol | His tag |
|---|---|
| Gene Summary | Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent′s anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells. |
| Application Notes | capacity:≥0.6 mg/mL, gel binding capacity |
|---|
| Form | suspension |
|---|---|
| Storage Instructions | For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze. |
| Storage Buffer | Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide |
